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Papers of the Week

Papers: 25 Feb 2023 - 3 Mar 2023

Basic Science

In Vitro Studies, Molecular/Cellular, Pharmacology/Drug Development

Arthritis, Inflammation/Inflammatory

2023 Feb 08

Antioxidants (Basel)




Hydrogen Sulfide Downregulates Oncostatin M Expression via PI3K/Akt/NF-κB Signaling Processes in Neutrophil-like Differentiated HL-60 Cells.


Han NR, Ko SG, Park HJ, Moon PD


The cytokine oncostatin M (OSM) is regarded as a critical mediator in various inflammatory responses. While the gaseous signaling molecule hydrogen sulfide (HS) plays a role in a variety of pathophysiological conditions, such as hypertension, inflammatory pain, osteoarthritis, ischemic stroke, oxidative stress, retinal degeneration, and inflammatory responses, the underlying mechanism of HS action on OSM expression in neutrophils needs to be clarified. In this work, we studied how HS reduces OSM expression in neutrophil-like differentiated (d)HL-60 cells. To evaluate the effects of HS, sodium hydrosulfide (NaHS, a donor that produces HS), ELISA, real-time PCR (qPCR), immunoblotting, and immunofluorescence staining were utilized. Although exposure to granulocyte-macrophage colony-stimulating factor (GM-CSF) resulted in upregulated levels of production and mRNA expression of OSM, these upregulated levels were reduced by pretreatment with NaHS in dHL-60 cells. Similarly, the same pretreatment lowered phosphorylated levels of phosphatidylinositol 3-kinase, Akt, and nuclear factor-kB that had been elevated by stimulation with GM-CSF. Overall, our results indicated that HS could be a therapeutic agent for inflammatory disorders via suppression of OSM.