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Papers of the Week


Papers: 22 Mar 2025 - 28 Mar 2025


2025 Mar 23


J Neurosci Methods


40132688

A comprehensive protocol for simplified mouse DRG fixation, processing and F4/80 immunohistochemistry: Overcoming common challenges.

Authors

Tay N, Alshammari A, Kaur S, Pettit A, Mu E, Reid A, Winkler I, Vetter I, Starobova H

Abstract

Dorsal root ganglia (DRGs) contain the cell bodies of sensory neurons and non-neuronal cells that play a role in the pathophysiology of painful inflammatory conditions, such as neuropathic pain. Immunohistochemistry (IHC) is a valuable tool for visualising and quantifying immune cell markers in DRGs, providing important insights into these mechanisms. However, isolating DRGs while preserving cell morphology for IHC staining is technically challenging due to their small size and location within the spinal column.