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Papers of the Week

Papers: 17 Sep 2022 - 23 Sep 2022

2022 Sep 22

Exp Dermatol

RNA sequencing reveals the transcriptome profile of the atopic prurigo nodularis with severe itching.


Shao Y, Zhu Y, Xiao Z, Shen Y, Dai B, Tang H, Wang D
Exp Dermatol. 2022 Sep 22.
PMID: 36134503.


Prurigo nodularis (PN), characterised by inevitable chronicity and severe pruritus, is most frequently associated with atopy compared to other origins. However, the skin transcriptomic profiling of PN arising from atopic dermatitis (AD), so-called atopic PN (APN), remains unclear. We sought to explore the cutaneous transcriptome of APN with severe pruritus and compare it to classic AD. RNA sequencing was performed on the lesional skin from 13 APN and 11 AD patients with severe pruritus (itch numerical rating scale score ≥7) and normal skin from 11 healthy subjects. Quantitative real-time polymerase chain reaction and immunochemistry were used for validation. We detected 1085 and 1984 differentially expressed genes (DEGs) in lesional APN skin and lesional AD skin versus normal skin, respectively. In total, 142 itch/inflammation-related DEGs were identified. Itch/inflammation-related DEGs, such as IL-6, IL-10, IL-13, oncostatin M, and IL-4 receptor, had elevated gene transcript levels in both diseases. The itch/inflammation-related DEGs that increased only in APN were mainly neuroactive molecules, while many inflammatory mediators such as T helper 22-related genes were found to be increased only in AD. Both disorders showed mixed Th1/Th2/Th17 polarisation and impaired skin barrier. In contrast to AD, M1/M2 macrophage activation, tumour necrosis factor production, fibrosis, revascularization and neural dysregulation are unique features of APN. The study findings broaden our understanding of the pathogenesis underlying APN, which provides insights into novel pathogenesis with potential therapeutic implications.