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Papers of the Week

Papers: 24 Feb 2024 - 1 Mar 2024






20-HETE mediated TRPV1 activation drives allokinesis via MrgprA3 neurons in chronic dermatitis.


Yu G, Liu P, Huang X, Qi M, Li X, Feng W, Shang E, Zhou Y, Wang C, Yang Y, Zhu C, Wang F, Tang Z, Duan J


Noxious stimuli are often perceived as itchy in patients with chronic dermatitis (CD); however, itch and pain mechanisms of CD are not known. TRPV1 involvement in CD was analyzed using a SADBE induced CD-like mouse model, and several loss- and gain-of-function mouse models. Trigeminal TRPV1 channel and MrgprA3 neuron functions were analyzed by calcium imaging and whole-cell patch-clamp recordings. Lesional CD-like skin from mice were analyzed by unbiased metabolomic analysis. 20-HETE availability in human and mouse skin were determined by LC/MS and ELISA. And finally, HET0016, a selective 20-HETE synthase inhibitor, was used to evaluate if blocking skin TRPV1 activation alleviates CD-associated chronic itch or pain. While normally a pain inducing chemical, capsaicin induced both itch and pain in mice with CD condition. DREADD silencing of MrgprA3 primary sensory neurons in these mice selectively decreased capsaicin induced scratching, but not pain-related wiping behavior. In the mice with CD condition, MrgprA3 neurons showed elevated ERK phosphorylation. Further experiments showed that MrgprA3 neurons from mice, which have constitutively active BRAF in MrgprA3 neurons, were significantly more excitable and responded more strongly to capsaicin. Importantly, capsaicin induced both itch and pain in mice in an MrgprA3 neuron dependent manner. Finally, the arachidonic acid metabolite 20-HETE, which can activate TRPV1, was significantly elevated in the lesional skin of mice and patients with CD. Treatment with the selective 20-HETE synthase inhibitor HET0016 alleviated itch in mice with CD condition. Our results demonstrate that 20-HETE activates TRPV1 channels on sensitized MrgprA3 neurons, and induces allokinesis in lesional CD skin. Blockade of 20-HETE synthesis or silencing of TRPV1-MrgprA3 neuron signaling offers promising therapeutic strategies for alleviating CD-associated chronic itch.