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Oxid Med Cell Longev


Intraperitoneal Lavage with Prevents Postoperative-Induced Peritoneal Adhesion in a Rat Model: Evidence from Animal and Cellular Studies.


Rahmanian-Devin P, Rakhshandeh H, Rahimi V B, Sanei-Far Z, Hasanpour M, Memarzia A, Iranshahi M, Askari V R
Oxid Med Cell Longev. 2021; 2021:5945101.
PMID: 34956439.


Postoperative peritoneal adhesions are considered the major complication following abdominal surgeries. The primary clinical complications of peritoneal adhesion are intestinal obstruction, infertility, pelvic pain, and postoperative mortality. In this study, regarding the anti-inflammatory and antioxidant activities of we aimed to evaluate the effects of on the prevention of postsurgical-induced peritoneal adhesion. Male Wistar-Albino rats were used to investigate the preventive effects of extract (0.5%, 0.25% and 0.125% /) against postsurgical-induced peritoneal adhesion compared to pirfenidone (PFD, 7.5% /). We also investigated the protective effects of PFD (100 g/ml) and extract (100, 200, and 400 g/ml) in TGF-1-induced fibrotic macrophage polarization. The levels of cell proliferation and oxidative, antioxidative, inflammatory and anti-inflammatory, fibrosis, and angiogenesis biomarkers were evaluated both and models. extract ameliorates postoperational-induced peritoneal adhesion development by attenuating oxidative stress [malondialdehyde (MDA)]; inflammatory mediators [interleukin- (IL-) 6, tumour necrosis factor- (TNF-) , and prostaglandin E (PGE)]; fibrosis [transforming growth factor- (TGF-) 1, IL-4, and plasminogen activator inhibitor (PAI)]; and angiogenesis [vascular endothelial growth factor (VEGF)] markers, while propagating antioxidant [glutathione (GSH)], anti-inflammatory (IL-10), and fibrinolytic [tissue plasminogen activator (tPA)] markers and tPA/PAI ratio. In a cellular model, we revealed that the extract, without any toxicity, regulated the levels of cell proliferation and inflammatory (TNF-), angiogenesis (VEGF), anti-inflammatory (IL-10), M1 [inducible nitric oxide synthase (iNOS)] and M2 [arginase-1 (Arg 1)] biomarkers, and iNOS/Arg-1 ratio towards antifibrotic M1 phenotype of macrophage, in a concentration-dependent manner. Taken together, the current study indicated that reduces peritoneal adhesion formation by modulating the macrophage polarization from M2 towards M1 cells.