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Papers of the Week

Papers: 19 Jun 2021 - 25 Jun 2021

Human Studies, Pharmacology/Drug Development

2021 Jun 18

STAR Protoc



Protocol for crystal structure determination of the antagonist-bound human cannabinoid receptor CB2.


Shen L, Li X, Liu J, Liu K, Liu Z-J, Hua T
STAR Protoc. 2021 Jun 18; 2(2):100584.
PMID: 34151302.


Human cannabinoid receptor CB2 plays an important role in the immune system and is an attractive therapeutic target for pain and for inflammatory and neurodegenerative diseases. However, the structural basis of CB2 agonist selectivity is still elusive. Here, we describe a detailed protocol for the determination of the crystal structure of antagonist AM10257-bound CB2. This methodology could be applied to the structural studies of CB2 with diverse antagonists and agonists or to other class A G-protein-coupled receptors. For complete details on the use and execution of this protocol, please refer to Li et al. (2019).