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Papers of the Week

2020 Apr 20

Comp Med

Functional Deficits in Mice Expressing Human Interleukin 8.


Brent J M, Tian Z, Yao L, Huang J, Markova DZ, Shofer FS, Brice AK, Qin L, Scanzello CR, Vitale F, Chen D, Zhang Y
Comp Med. 2020 Apr 20.
PMID: 32312361.


We showed previously that inflammatory mediators, including IL8, in intervertebral disc tissues from patients with discogenicback pain may play a key role in back pain. To investigate the molecular mechanism of IL8 signaling in back pain, we generated a mouse model that conditionally expresses human (h) IL8. We hypothesized that hIL8 levels affect mouse activity and function. Briefly, hIL8 cDNA was inserted into the pCALL2 plasmid, linearized, and injected into mouse embryos. Resulting pCALL2-hIL8 mice were then bred with GDF5-Cre mice to express the transgene in cartilage and intervertebral disc(IVD) tissues. Functional capacities including nest-making and other natural behaviors were measured. Both male and female mice expressing hIL8 showed lower nesting scores than did littermates that did not express hIL8 (n = 14 to 16 per group). At 28 wk of age, mice expressing hIL8 (n = 35) spent more time immobile and eating during each night than littermate controls (n = 33). Furthermore, hIL8-expressing mice traveled shorter distances and at a lower average speed than littermate controls. Thus, in an initial effort to investigate the relationship between this chemokine and mouse behavior, we have documented changes in normal activities in mice conditionally expressing hIL8.