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Papers of the Week

Papers: 27 Apr 2019 - 3 May 2019


Methods Mol Biol


High-Content Imaging of Immunofluorescently Labeled TRPV1-Positive Sensory Neurons.


Isensee J, Hucho T
Methods Mol Biol. 2019; 1987:111-124.
PMID: 31028677.


Studying TRP channel expressing nociceptors requires the identification of the respective subpopulations as well as the quantification of dynamic cellular events. However, the heterogeneity of sensory neurons and associated nonneuronal cells demands the analysis of large numbers of cells to reflect the distribution of entire populations. Here we report a detailed workflow how to apply high-content screening (HCS) microscopy to signaling events in TRPV1-positive neurons as well as an approach to use the selective elimination of TRPV1 positive cells from dissociated rat sensory ganglia as base for transcriptomic analysis of TRPV1-positive cells and/or as control for TRPV1 antibody specificity.